Cloning, sequence, and expression of a blood group B active recombinant α-D-galactosidase from pinto bean (Phaseolus vulgaris)
نویسندگان
چکیده
منابع مشابه
Backcross breeding for improved resistance to common bacterial blight in pinto bean (Phaseolus vulgaris
Common bacterial blight (CBB) caused by Xanthomonas campestris pv. phaseoli reduces common bean (Phaseolus vulgaris L.) yield and quality worldwide. Genetic resistance provides effective disease control; however, a high level of resistance is difficult to attain and does not exist in pinto bean, the most important dry bean market class in North America. Our objective was to determine if a backc...
متن کاملExpression Cloning of Recombinant Escherichia coli lacZ Genes Encoding Cytoplasmic and Nuclear P-galactosidase Variants
Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...
متن کاملCloning and Expression of Recombinant Helicobacter pylori Urease A and B Subunits as a Putative Vaccine
Helicobacter pylori infection is among the most prevalent infections in the world involving more than half the adult population. H. pylori infection results in active chronic gastritis, peptic ulcers and enhances the risk of gastric malignancies. It is of utmost importance to prevent H. pylori infection particularly in highly prevalent countries including Iran. The urease holoenzyme produced by...
متن کاملEffect of Irrigation Regimes on Yield, Yield Components and Seed Quality of Different Pinto Bean (Phaseolus vulgaris) Genotypes
Identification of drought-resistant genotypes is crucial in order to reduce the negative effects of drought, as one of the most important limiting factors of crop production in arid and semiarid regions. Thus, effects of three irrigation regimes (irrigation after 50 (I1), 100 (I2) and 150 (I3) mm evaporation from a class A pan), as the main plot were investigated. These effects were studied on ...
متن کاملHeterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae
BACKGROUND Phaseolamin or α-amylase inhibitor 1 (αAI) is a glycoprotein from common beans (Phaseolus vulgaris L.) that inhibits some insect and mammalian α-amylases. Several clinical studies support the beneficial use of bean αAI for control of diabetes and obesity. Commercial extracts of P. vulgaris are available but their efficacy is still under question, mainly because some of these extracts...
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ژورنال
عنوان ژورنال: IUBMB Life
سال: 1997
ISSN: 1521-6543
DOI: 10.1080/15216549700202861